Journal: Neural Regeneration Research

Article Title: Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury ★

doi: 10.3969/j.issn.1673-5374.2012.10.004

Figure Lengend Snippet: Neurotrophic factor expression in surrounding injured brain tissues following human umbilical cord blood mesenchymal stem cell transplantation. (A–C) Nerve growth factor (NGF) protein expression (immunohistochemistry, × 200); (D–F) brain-derived neurotrophic factor (BDNF) protein expression (immunohistochemistry, × 200); (G–I) BDNF mRNA expression ( in situ hybridization, × 400). (A, D, G) Results (absorbance) are expressed as mean ± SD from six rats in each group at each time point. a P < 0.05, vs . model group ( t -test was used to specify differences between two groups at the corresponding time points); (B, E, H) model group at 14 days after transplantation; (C, F, I) transplantation group at 14 days after transplantation. Arrows: NGF protein-, BDNF protein-, and BDNF mRNA-positive cells.

Article Snippet: Primary antibodies were incubated overnight at 4°C in wet box with the following dilutions: rat anti-human BrdU monoclonal antibody (1:150; Beijing Boaosen Biotechnology, China), rabbit anti-mouse BDNF (1:150; Boster, Wuhan, China), NGF (1:150; Boster), VEGF (1:150; Boster), CD34 (1:100; Boster), bFGF (1:100; Beijing Boaosen Biotechnology) polyclonal antibody.

Techniques: Expressing, Transplantation Assay, Immunohistochemistry, Derivative Assay, In Situ Hybridization

Journal: Clinical and Experimental Immunology

Article Title: The effects of maternal anti-alpha-enolase antibody expression on the brain development in offspring

doi: 10.1093/cei/uxac086

Figure Lengend Snippet: quantitative examination of glial cells in hippocampal and cortical tissues and assessment of cytokine and BDNF levels in the brain tissue of the pups from H-ENO1Ab dams. All the pups were obtained as depicted in . The expression levels of Iba-1 (A) and GFAP (B) in the hippocampal and cortical tissues from the pups of H-ENO1Ab dams and those of the control group on P10 and P40 ( n = 4/group, respectively) were quantitatively assessed by western blot. * P < 0.05 vs. the pups of the control group at the same age. (C) The average levels of cytokines in the supernatants of brain tissue homogenates from the pups of H-ENO1Ab dams and those of the control group on P10 were detected by microarray ( n = 4/group). NS vs. the pups of the control group. (D) The levels of BDNF in the brain tissues of the pups from H-ENO1Ab dams and those of the control group on P10 ( n = 4/group) were detected by western blot. * P < 0.05 vs. the pups of the control group on P10.

Article Snippet: The membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ENO1 antibodies (1:400, BIOSS), rabbit anti-Iba-1 antibodies (1:1000, Abcam), mouse anti-GFAP antibodies (1:1000, CST), rabbit anti-cleaved caspase-3 antibodies (1:1000, CST), rabbit anti-BDNF antibodies (1:500, Wanleibio, China), and rabbit anti-beta-actin antibodies (1:5000, BIOSS).

Techniques: Expressing, Control, Western Blot, Microarray